The Mechanism of Antimicrobial Activity of Conjugated Bile Acids against Lactic Acid Bacilli

The mechanism underlying antimicrobial activity of conjugated bile acids against strains lactic acid bacilli is not well understood. purpose this study was to investigate two typical (glycochenodeoxycholic and taurochenodeoxycholic acid) for their mechanisms four different species at the physiological pH small intestine humans. bacterial cell membrane integrity, transmembrane potential, gradient were examined using fluorescence probes SYTO 9 plus propidium iodide, 3,3′-dipropylthiadicarbocyanine 5(6)-carboxyfluorescein diacetate N-succinimidyl ester, respectively. intracellular ATP levels measured by firefly luciferase-based bioluminescence method. It found that strain-specific, glycochenodeoxycholic showed significantly greater than bacilli. inhibited growth disrupting dissipating reducing gradient, depleting ATP. In conclusion, a multifactorial phenomenon. This will provide valuable information developing strategies improve ability tolerate in vivo.